Myriocin

Research use only, not for human use.

Myriocin, a fungal metabolite isolated from Myriococcum albomyces, Isaria sinclairi and Mycelia sterilia, is a potent inhibitor of serine-palmitoyl-transferase (SPT) and a key enzyme in de novo synthesis of sphingolipids.

Myriocin, a fungal metabolite isolated from Myriococcum albomyces, Isaria sinclairi and Mycelia sterilia, is a potent inhibitor of serine-palmitoyl-transferase (SPT) and a key enzyme in de novo synthesis of sphingolipids. Myriocin strongly suppresses replication of both the subgenomic HCV-1b replicon and the JFH-1 strain of genotype 2a infectious HCV, with an IC50 of 3.5 μg/mL for inhibiting HCV infection.

Myriocin (Thermozymocidin; 0-10000 nM; 96 h; Huh7/Rep-Feo cells) inhibits the replication of a subgenomic hepatitis C virus (HCV) genotype 1b replicon.Myriocin (100 nM; 72 h) decreases in the sphingomyelin content of Huh7 cells. Western Blot Analysis Cell Line:Huh7/Rep-Feo cells Concentration:0, 300, and 1000 nM Incubation Time:96 hours Result:Decreased the levels of HCV NS5A in a concentration-dependent manner.

Myriocin (Thermozymocidin; 0-1 mg/kg; i.p.; daily, for 5 d; male Balb/c mice) blocks de novo synthesis of sphingolipids in vivo by SPT inhibition and induced c-myc expression in liver. Animal Model:Male Balb/c mice (22 g, 7-8 weeks old)Dosage:0, 0.1, 0.3, and 1.0 mg/kg Administration:Intraperitoneal injection; daily, for 5 days Result:Altered not significantly the levels of plasma alanine aminotransferase and aspartate aminotransferase. Decreased free sphinganine in a dose-dependent manner in both the liver and kidney.Decreased in the activity of liver SPT with a correlation coefficient of -0.76.

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Microbiology/Virology

HCV

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35891-70-4

401.54

C??H??NO?

>98% (HPLC)

DMSO : 5 mg/mL (12.45 mM; ultrasonic and warming and heat to 60°C)

CCCCCCC(CCCCCC/C=C/C[C@@H](O)[C@H](O)[C@](CO)(N)C(O)=O)=O

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(-20℃)

With Ice Pack

≥ 2 years